Functional Studies of FIN219 and FIN219-interacting Protein 2 (FIP2) Interaction in the Integration of Jasmonate, Abscisic Acid and Light Signaling in Arabidopsis
Date Issued
2014
Date
2014
Author(s)
Chang, Chin-Wen
Abstract
Light is one of the most important environmental factors regulating multiple aspects of plant growth and development. Abscisic acid (ABA) and jasmonate (JA) are plant hormones that play important roles during many phases of the plant life cycle and in plant responses to various environmental stresses. How plants integrate the external light signal and endogenous ABA and JA pathways for better adaptation to environmental stresses remains unknown. Far-red insensitive 219 (FIN219/JAR1) has been shown to be a positive signal component in FR signaling pathway. To further understand the function of FIN219 in light signaling during Arabidopsis development, a yeast two-hybrid approach has been used to isolate FIN219-interacting partners. A gene FIP2 (FIN219-interacting protein 2) encoding a glutamyl-tRNA synthetase (GluRS) was isolated. However, its function is not completely understood although previous studies showed that GluRS interacted with ABA INSENSITIVE 1 (ABI1) and Homeodomain protein 6 (AtHB6). By Co-IP Assays, we demonstrate a physical interaction between the full-length FIN219 and FIP2 in Nicotiana benthamiana, and this interaction occurred via the C terminus of FIN219 and the FIP2 in Arabidopsis thaliana. Besides, under FR and MeJA treatments, FIN219 and FIP2 can negatively regulate each other. Furthermore, the fip2 mutant shows insensitivity in hypocotyl growth to MeJA and ABA under FR light. Moreover, FIP2 overexpression results in the insensitivity of stomatal opening to MeJA and ABA. Interestingly, both FIP2 knocked-down mutant and overexpressor lines showed a defect in the number and the development of chloroplasts. Taken together, these data indicate that FIP2 may function via different mechanisms from shoot to root in ABA signaling and participate in the integration of FR light, JA and ABA signaling in Arabidopsis.
Subjects
光
離層酸
茉莉酸
Type
thesis
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