A Simple and Cost-Saving Approach To Optimize the Production of Subtilisin NAT by Submerged Cultivation of Bacillus subtilis Natto
Resource
Journal of Agricultural and Food Chemistry 57 (1): 292-296
Journal
Journal of Agricultural and Food Chemistry
Journal Volume
57
Journal Issue
1
Pages
292-296
Date Issued
2009
Date
2009
Author(s)
Abstract
Subtilisin NAT, formerly designated nattokinase or subtilisin BSP, is a potent cardiovascular drug because of its strong fibrinolytic activity and safety. In this study, one Bacillus subtilis natto strain with high fibrinolytic activity was isolated. We further studied the optimal conditions for subtilisin NAT production by submerged cultivation and three variables/three levels of response surface methodology (RSM) using various inoculum densities, glucose concentrations, and defatted soybean concentrations as the three variables. According to the RSM analysis, while culturing by 2.93% defatted soybean, 1.75% glucose, and 4.00% inoculum density, we obtained an activity of 13.78 SU/mL. Processing the batch fermentation with this optimal condition, the activity reached 13.69 SU/mL, which is equal to 99.3% of the predicted value.
Type
journal article
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