Cloning and characterization of the endogenous retroviral-tRNAGlu multigene family from human genomes of different racial backgrounds
Resource
Gene 155 (2): 247-252
Journal
Gene
Pages
247-252
Date Issued
1995
Date
1995
Author(s)
Yeh, Kai-Wun
Yang, Wen K.
Huang, Huey-Chung
Feng, Ya-Nan
Liu, Jau-Ching
Wu, Felicia Y. -H.
Wu, Cheng-Wen
Abstract
An 8.3-kb human endogenous retroviral-tRNAGlu (HERV-E)-encoding cDNA clone and a 1.5-kb genomic clone were isolated from a Chinese-derived cervical cancer cell line, CC7T, and their sequences determined. The former is a full-length endogenous retroviral cDNA containing corresponding u5-gag-pol-env-u3-r regions. The latter is a partial retroviral DNA segment, covering the gag and pol genes. Analysis of normal human DNA by Southern blot hybridization with three specific HERV-E molecular DNA probes revealed complex restriction-fragment length polymorphisms (RFLP), implying that the human genome contains diverse proviral structures and dispersed integration sites. The complex patterns were virtually identical between DNAs from African-Americans, Asians and Caucasians, with only a few minor variations. The data suggest that these proviral sequences were mostly incorporated into the human genome before racial divergence and, hence, may serve as markers for distinct chromosomal sites. ? 1995.
Subjects
Proviral structure; restriction-fragment length polymorphism; transduced sequence; truncated structure
SDGs
Other Subjects
transfer rna; article; cancer cell; cell line; genome; human; human cell; multigene family; nonhuman; nucleotide sequence; priority journal; provirus; race; retrovirus; Amino Acid Sequence; Cell Line, Transformed; Cloning, Molecular; Genome; Human; Molecular Sequence Data; Racial Stocks; Retroviridae; RNA, Transfer, Glu; RNA, Viral; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.
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