Fibrinogen Gel Mixed with Platelet-rich Fibrin as Scaffold Seeded with Swine Dental Bud Cell for Tooth Regeneration
Date Issued
2009
Date
2009
Author(s)
Wang, Chun-Hao
Abstract
Loss of teeth has many causes including physical trauma, gum disease and tooth decay. A biological tooth substitute that could replace lost teeth would provide a vital alternative to currently available clinical treatments. The purpose of this project is to generate a complete tooth crown by tissue engineering. Platelet-rich fibrin(PRP) is a new type of platelet concentrate which can apply a lot of growth factors and morphogens in osteogenesis, including platelet-derived growth factor（PDGF）、bone morphogenetic protein (BMP)、epidermal growth factor（EGF）、transforming growth factor-beta (TGF-β)、and vascular endothelial growth factor (VEGF). Partial of those growth factors and morphogens which involve in tooth development are match with odontogenesis. The aim of this project is to combined the dental bud cells (DBC)、fibrinogen gel and PRF to regenerate a complete tooth. The dental bud cells of molar tooth were removed from 1.5 month-old miniature pigs before eruption. When the DBC yield reached 1×106 cell/ml, the DBC were isolated, re-suspended, and then mixed the cell with fibrinogen gel and PRF. The DBC/fibrinogen gel/PRF constructs were implanted in xenograft into under dermis of nude mice’s pelvic dorsum. After xenogreat implantation for 16 weeks, there were evaluated by macrography and microsections stained with hematoxylin and eosin. We could see mineralized nodule, dentinogenic-like cells and vascular. In the swine experimental groups, one of the 9 pigs generated root, pulp, enamel, ameloblast, dentin, odontoblast, dentin tubular, cementum, blood vessels, periodontal ligament, and the other one pig erupted the regeneration tooth, and generated whole crown, root, pulp, enamel, dentin, odontoblast, dentin tubular, cementum, blood vessels, periodontal ligament. The immunohistochemical analysis results showed ameloblast expressed CK14, the dentin, dentin tubular and odontoblast expressed DMP-1, cementum and alveolar bone expressed OPN and the vascular endothelial cell of pulp expressed VEGF, too. We demonstrated that the porcine dental bud cells combined with fibrinogen gel and PRF which can grow regenerated tooth by tissue engineering when porcine model with autogenic cell transplantation.
Subjects
dental bud cell
platelet-rich fibrin
tissue engineering
tooth regeneration
nude mice
mini pig
Type
thesis
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