Isolation and Characterization of Nonfermentative Facultative Anaerobic Bacteria from Shiangshan Wetland
Date Issued
2008
Date
2008
Author(s)
Liao, Chu-Yu
Abstract
Coastal wetland is a transitional zone of land margin and water bodies. When wetland was inundated, respiring microorganisms quickly consume oxygen that is dissolved in the water or trapped in the soil. This causes suboxic or even anaerobic conditions. Nonfermentative facultative anaerobic bacteria can use either oxygen or other terminal electron acceptors such as nitrate (NO3-), oxidized iron (Fe3+), manganese (Mn4+) and sulfate (SO42-) and then cause nitrogen cycle, iron cycle, manganese cycle, and even sulfur cycle. They play an important role in mineral cycles.he samples were obtained from the coastal ecosystem near Koya Water Resource Recycling Center and Haishangu during years 2007 and 2008. After serial dilution, the samples were injected into PYG (Peptone-Yeast-Glucose) broth medium, and then incubated under anaerobic condition. The abundance of nonfermentative facultative anaerobic bacteria were evaluated by the method of most-probable-number (MPN) in PYG broth, and the MPN values were ranging from 3.4×104～2.4×106 cells /ml.orty-five nonfermentative isolates are obtained from PYG (Peptone-Yeast-Glucose) broth medium. We use streak plate method to get pure culture and then stored pure isolates in PY stab. There are thirty-one denitrifying isolates. In addition, it suggested most of nonfermentative facultative anaerobes are denitrifying bacteria according to the counting values of denitrifiers (3×104～9.2×106 cells/ml) and nonfermentative facultative anaerobes (3.4×104～2.4×106 cells /ml) in this study. orty-five isolates are divided into twelve groups, according to the restriction fragment length polymorphisms (RFLP) analysis with three endonucleases, RsaI, DdeI and AluI. These bacteria are belonging to eight genera, among them Marinobacter is the major, according to phylogenetic analysis of 16S rDNA sequences of representative isolates.he phenotypic properties of C19 are more similar to Pseudoalteromonas haloplanktis than to the most similar species. However, the C19 shows low 16S rDNA identity of 97.8 % with Pseudoalteromonas haloplanktis. It is necessary to study futhur to tell whether C19 is a novel species or not.
Subjects
Shiangshan Wetland
Facultative Anaerobic Bacteria
RFLP
Type
thesis
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