蝴蝶蘭基因體研究計畫─以核糖體RNA基因為蝴蝶蘭屬植物的分子標誌
Date Issued
2005
Date
2005
Author(s)
高燕玉
DOI
922317B002021
Abstract
18S-5.8S-26S (45S) and 5S rDNAs are
organized into clusters of tandem repeat with
each repeat consisting of a transcribed region,
and an intergenic spacer (IGS). In the
transcribed region of 45S rDNA, on either side
of 5.8S rDNA are found internal transcribed
spacers (ITS), described as ITS1 and ITS2. The
ITS and IGS have sequences diverged more
than the transcribed region, and the presence of
highly conserved sequences flanking each of
ITS and IGS make them easy to amplify and
useful for phylogenetic analyses of interspecies.
In this study, the IGS sequences of 28
Phalaenopsis species and one Doritis species
were amplified by the polymerase chain
reaction (PCR) which used specific primers
devised by 5S rDNA transcribed region of P.
aphrodite. The neighbor-joining tree
constructed from IGS consensus sequences
divides the 28 Phalaenopsis species into seven
groups. The species from four groups coincided
with the results from the IGS and ITS
sequences analyses. Additionally, species in
IGS phylogenetic tree were in agreement with
traditional taxonomic status.
Subjects
Phalaenopsis
IGS
phylogeny
Publisher
臺北市：國立臺灣大學分子與細胞生物學研究所
Type
report
File(s)![Thumbnail Image]()
Loading...
Name
922317B002021.pdf
Size
159.98 KB
Format
Adobe PDF
Checksum
(MD5):ef892035eefd7c483553299528f468b0