Structure identification of aporphine alkaloids by on-line coupling of HPLC-NMR with loop storage
Journal
Journal of the Chinese Chemical Society
Journal Volume
47
Journal Issue
6
Pages
1231-1236
Date Issued
2000
Author(s)
Abstract
The potential of coupling HPLC separation methodology to on-line high resolution nuclear magnetic resonance (NMR) spectroscopy has been demonstrated with a mixture of nine aporphine alkaloids. A loop storage procedure after separation has facilitated the identification of closely eluted peaks (α = k2/k1 = 1.01 and Δv = 0.37 min = 367 μL). These were collected off-line and identified by a 1D 1H NMR spectrum. For this purpose an automated procedure has been implemented which includes the use of shaped pulses, multiple solvents suppression and 13C satellite suppression of acetonitrile.
SDGs
Type
journal article