Development of Electrode-Assisted Desorption Electrospray Ionization and Its biomedical Applications
Date Issued
2010
Date
2010
Author(s)
Wang, Yu-Wen
Abstract
Ambient ionization mass spectrometry has recently played an important role in Biological Mass Spectrometry because pre-treatment of samples is not required. It can be used for direct analysis of solid and liquid samples with a simple mass spectrometer. We developed the novel Electrode-Assisted Desorption Electrospray Ionization (EADESI) method for quick biomedical sample analysis. EADESI is a novel ambient ionization method which can be used as detection of samples on nearly any type of substrate. The instrument consists of three major parts: a three-dimensional movable sample plate with ground potential, a high voltage electrode, and a spray source. The spray source and electrode are positioned at about 45° with respect to each other and directly above the sample plate. Solvent nebulized by gas was used as a spray device to spray onto the sample, and then the desorbed analyte ions were introduced into the mass spectrometer. Our results indicated that EADESI can have space resolution of 25 μm. EADESI also has a wide dynamic range for different types of biomedical compounds.
Subjects
Ambient ionization
Electrode-Assisted Desorption Electrospray Ionization
File(s)![Thumbnail Image]()
Loading...
Name
ntu-99-R97223213-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):d00ad6d54f25fbe3ff9c8780cd602fae