Comprehensive Transcriptional Profiling and Mouse Phenotyping Reveals Dispensable Role for Adipose Tissue Selective Long Noncoding RNA Gm15551
Journal
Non-Coding RNA
Journal Volume
8
Journal Issue
3
Start Page
32
ISSN
2311-553X
Date Issued
2022-05-06
Author(s)
Christoph Andreas Engelhard
Sajjad Khani
Petr Kasparek
Jan Prochazka
Jan Rozman
David Pajuelo Reguera
Radislav Sedlacek
Jan-Wilhelm Kornfeld
Abstract
Cold and nutrient‐activated brown adipose tissue (BAT) is capable of increasing systemic energy expenditure via the uncoupled respiration and secretion of endocrine factors, thereby protecting mice against diet‐induced obesity and improving insulin response and glucose tolerance in men. Long non‐coding RNAs (lncRNAs) have recently been identified as fine‐tuning regulators of cellular function. While certain lncRNAs have been functionally characterised in adipose tissue, their overall contribution in the activation of BAT remains elusive. We identified lncRNAs correlating to interscapular brown adipose tissue (iBAT) function in a high fat diet (HFD) and cold stressed mice. We focused on Gm15551, which has an adipose tissue specific expression profile, is highly upregulated during adipogenesis, and downregulated by β‐adrenergic activation in mature adipocytes. Although we performed comprehensive transcriptional and adipocyte physiology profiling in vitro and in vivo, we could not detect an effect of gain or loss of function of Gm15551.
Subjects
dipose tissue remodelling
brown adipose tissue
long noncoding RNAs
Publisher
MDPI AG
Description
Article number 32
Type
journal article