Cytotoxic and anti-inflammatory metabolites from the soft coral Scleronephthya gracillimum
Journal
Marine Drugs
Journal Volume
11
Journal Issue
6
Pages
1853-1865
Date Issued
2013
Author(s)
Abstract
Five new pregnane-type steroids, sclerosteroids J-N (1-5), and a diterpenoid with a new chemotype 3-methyl-5-(10′-acetoxy-2′, 6′,10′-trimethylundecyl)-2-penten-5-olide (6), have been isolated from a soft coral Scleronephthya gracillimum. The structures of the metabolites were determined by extensive spectroscopic analysis. Compound 4 exhibited cytotoxicity against HepG2, A549, and MDA-MB-231 cancer cell lines. Furthermore, steroids 2 and 4 were found to significantly inhibit the accumulation of the pro-inflammatory iNOS protein, and 1, 2, 4 and 5 could effectively reduce the accumulation of COX-2 protein in LPS-stimulated RAW264.7 macrophage cells. ? 2013 by the authors; licensee MDPI.
Subjects
Anti-inflammatory activity; Cytotoxicity activity; Scleronephthya gracillimum; Soft coral
Other Subjects
3 methyl 5 (10' acetoxy 2' ,6' ,10' trimethylundecyl) 2 penten 5 olide; a beta (2' o acetyl alpha fucopyranosyloxy) 5 alpha pregna 20 en 19 al; antiinflammatory agent; cyclooxygenase 2; diterpenoid; pregnane derivative; sclerosteroid; unclassified drug; antiinflammatory activity; article; cancer cell culture; carbon nuclear magnetic resonance; controlled study; coral; cytotoxicity test; drug cytotoxicity; drug isolation; drug structure; high performance liquid chromatography; human; human cell; immunoblotting; macrophage; melting point; nuclear Overhauser effect; proton nuclear magnetic resonance; Scleronephthya gracillimum; Alcyonacea; Scleronephthya gracillimum
Type
journal article