Effects of pre-irradiation and SDF-1 suppression on the progression of murine astrocytoma cells grown in different stromal beds
Journal
International Journal of Radiation Biology
Journal Volume
90
Journal Issue
12
Pages
1162-1168
Date Issued
2014
Author(s)
Abstract
Purpose: To examine whether brain tumors grown in pre-irradiated (PreIR) thigh have a similar tumor bed effect (TBE) as in PreIR brain tissue.Material and methods: Tumor growth delay and immunohistochemical (IHC) staining for CD31, an endothelial surface marker, and PIMO, a hypoxia marker, were used to study the TBE of a murine astrocytoma, ALTS1C1, or a stromal-derived factor-1 (SDF-1) gene-silenced astrocytoma, ALTS1C1-SDFkd, growing in different PreIR stroma beds.Results: ALTS1C1 tumors growing in both PreIR brain and PreIR thigh had reduced microvascular density (MVD) and more chronic hypoxia, but tumor growth delay was only seen in PreIR brain tissue. In contrast, ALTS1C1-SDFkd tumors showed tumor growth delay in PreIR thigh, with little effect in PreIR brain tissue.Conclusions: This study cautions that both the tumor and the nature of the PreIR stromal bed are important when using pre-irradiation as a model of recurrent brain tumors after radiation therapy. ? 2014 Informa UK, Ltd.
Subjects
Glioma; Radiation therapy; SDF-1
SDGs
Other Subjects
CD31 antigen; chemokine receptor CXCR4; plerixafor; stromal cell derived factor 1; Cxcl12 protein, mouse; stromal cell derived factor 1; animal experiment; animal model; animal tissue; Article; astrocytoma; astrocytoma cell; brain radiation; brain tissue; cancer transplantation; cell growth; controlled study; gene repression; gene silencing; immunohistochemistry; microvasculature; mouse; nonhuman; survival time; thigh; tumor bed effect; tumor growth; tumor recurrence; tumor volume; animal; astrocytoma; brain; Brain Neoplasms; C57BL mouse; cell proliferation; cell transformation; deficiency; gene silencing; genetics; pathology; radiation response; stroma cell; tumor cell line; Murinae; Animals; Astrocytoma; Brain; Brain Neoplasms; Cell Line, Tumor; Cell Proliferation; Cell Transformation, Neoplastic; Chemokine CXCL12; Gene Silencing; Mice; Mice, Inbred C57BL; Stromal Cells; Thigh
Type
journal article