G Protein Beta 2 Subunit Antisense Oligonucleotides Inhibit Cell Proliferation and Disorganize Microtubule and Mitotic Spindle Organization
Resource
JOURNAL OF CELLULAR BIOCHEMISTRY v.83 n.1 pp.136-146
Journal
JOURNAL OF CELLULAR BIOCHEMISTRY
Journal Volume
v.83
Journal Issue
n.1
Pages
136-146
Date Issued
2001
Date
2001
Author(s)
WU, HAN-CHUNG
Abstract
The association of G protein beta2 subunit (G beta2) with mitotic spindles in various mammalian cells has been demonstrated previously. Recently, we have identified the association of G beta2 protein with microtubules (Wu et al., [1998] J. Cell. Biochem. 70: 552-562). In the present experiment we have demonstrated the possible functional role of G beta2 in microtubule and mitotic spindle organization in mammalian cells. When G beta2 antisense phosphorothioate oligonucleotides were transfected into mammalian cells, inhibition of cell proliferation with cell death after a 4- day treatment was observed. If the transfected cells were incubated for two days and their G beta2 and microtubules were examined by Western blotting and immunofluorescence localization, marked reduction of the G beta2 protein, fragmentation and disassembly of cytoplasmic microtubules, and disorganized mitotic spindles were found. We conclude that the G beta2 protein is closely associated with microtubule assembly and may play a potential role in the regulation of cell proliferation and microtubule and mitotic spindle organization in mammalian cells. J. Cell. Biochem. 83: 136-146, 2001. (C) 2001 Wiley-Liss, Inc.
Subjects
G beta 2 protein
antisense oligonucleotide transfection
microtubule assembly
mitotic spindle organization
nasopharyngeal carcinoma cells
BINDING REGULATORY PROTEIN