桿菌屬細菌表現系統之建立及應用─桿菌屬拮抗細菌之開發與改良(3/3)
Date Issued
1999
Date
1999
Author(s)
DOI
882316B002016B19
Abstract
Several chitinase - producing
bacteria are known to degrade the
fungal cell wall and are able to inhibit
the growth of fungi. Applications of
chitinase-producing bacteria in plant
disease control have been reported. In
this study, we transferred the chiA gene
of chitinase-producing bacterium,
Bacillus circulans, into the antagonist,
Bacillus subtilis and examined the
effect of ChiA1 protein on the
antagonistic activity of B. subtilis.
Firstly, the chiA gene was subcloned
with its own promoter into the shuttle
vector, pHY300PLK, and transferred
into the bacterial cells of Bacillus
subtilis by protoplast transformation.
The expression of ChiA1 protein was
detected in the supernatant of B. subtilis
culture, indicating that the ChiA1
2
protein is secreted out of the Bacillus
cell. The ChiA1 protein was detected
24 hr after cultivation of Bacillus in LB
broth and in a later period when
incubated in chitin medium. In
Escherichia coli cells, the ChiA1
protein is translocated into the
periplasmic space. The chitinase is
released from E. coli cells after
prolonged incubation. The expression
of chiA gene in a non-antagonistic
mutant of B. subtilis could display
inhibition effect on the growth of
Botrytis elliptica . However, when the
chiA gene was expressed in the
antagonistic strain of B. subtilis, the
enhancing effect of ChiA1 on the
antagonistic activity of B. subtilis was
not readily observed.
Publisher
臺北市：國立臺灣大學植物病理與微生物學系暨研究所
Type
other
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