Detection of C-Reactive Protein Using Resonance Micro-cantilever Beam Sensor
Date Issued
2010
Date
2010
Author(s)
Lin, Chung-An
Abstract
Resonant based micro-cantilever was applied for the detection of C-reactive protein based on the technology of fiber interferometry. The vast apparatus was simplified, and the stability was increased. Resonant frequency shift of the micro-cantilever measured by fiber interferometry was remained undisturbed during experimental procedure.C-reactive protein was immobilized on micro-cantilever using surface micromachining technique. Micro-cantilever was immersed in the solution containing molecules which were about to be bonded, and parched before measuring the resonant frequency. The crystalline solid from dried PBS on surface of the micro-cantilever will affect the measurement and cause erroneousness. The D.I. water washout process was employed to fix the issue. The concentrations of C-reactive protein solution were 1, 10, 100 and 1000 ug/mL, and total resonant frequency shift were 117.6, 144, 211 and 232.8 Hz respectively. A distinct change in the resonant frequency of micro-cantilever was observed as a function of time. Resonant frequency decreased rapidly over 70% from initial value in the first 30 minutes of C-reactive protein antigen-antibody interaction and attained 95% after 240 minutes in saturation.
Subjects
Bio-sensor
Resonant based micro-cantilever
fiber interferometry
Type
thesis
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