Rapid laser-assisted patterning of three heterotypic cell types on poly (vinyl alcohol) coated substrate for skin and hair follicle regeneration
Date Issued
2011
Date
2011
Author(s)
Lin, Meng-Wei
Abstract
Artificial skin has developed over twenty years, it could regenerate dermis and avoid scar formation. The neodermis could sense touch, cold and hot, but lack of appendages, like hair follicle and sudoriferous glands, and it is comlex to operate surgery on the scalp. From previous studies, we know dermal papillae could induce hair follicle regeneration, especially the aggregation form. It has developed many models to produce dermal papillae microtissues in vitro, but the efficiency and orientation are still low.
Our study uses CO2 laser to rapidly fabricate different patterns and confine dermal papillae to aggregate into the holes by poly(vinyl alcohol). Then pattern fibroblasts and keratinocytes with the time-window of PVA barrier and the adhesive ability of cells. The resultant model shows that only the microtissues in the hole have strong ability to induce hair follicles.
The model could apply in the research of interactions between more cells and the pattern of two mesenchymal cells. Furthermore, it could be a stage for future cell sheet implantation.
Subjects
skin regeneration
hair regeneration
laser
PVA
cell patterning
Type
thesis
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