Phylogeny and biological activity assay in Antrodia composite species
Date Issued
2005
Date
2005
Author(s)
Fan, Hsun-Fang
DOI
zh-TW
Abstract
o define the phylogenetic relationship of Antrodia and allied genera, the ITS1-5.8S-ITS2 rDNA or partial 18S and 28S rDNA sequences, which were either generated by our laboratory or retrieved from NCBI GeneBank database, were analyzed by MEGA 3 in combination with neighbor-joining algorithium. The constructed phylogenetic trees by 18S or 28S rDNA sequences showed that all the 14 Antrodia species were clustered in a monophyletic clade. The results did not endorse the recombination of A. cinnamonmea and A. salmonea to a newly erected Taiwanofungus genus. The phylogenetic analysis also indicated a more close relationship of Antrodia to Polyporaceae rather than Meripilaceae. Evidence also revealed that the HPLC chromatogram of mycelia methanol extract from Antrodia did not congruent with rDNA phylogenetic clade, though some of the unique peaks in the chromatograms may possibly serve as the markers of the 14 Antrodia species. Of the 16 Antrodia species tested, the methanolic extract of 11 species exhibited more than 50％ suppression activity toward Hep G2, while 7 species showed more than 50％ suppress effect toward A549. Nevertheless, all the Antrodia strains tested none of them exhibited marked suppression effect towarded the normal cell line NIH-3T3. Furthermore, the ethyl acetate fraction from A. cinnamomea mycelia also suppress the inflammatory response of mouse macrophage cell line（Raw 264.7） induced by lipopolysaccharide（LPS） at dosage of 500 ng/ml. In general, A. cinnamomea the mycelia ethyl acetate fractions were more potent than methanol fraction on their cancer cell suppress activity. Consequently, the active constituents in these fractions deserve to further purification and characterization.
Subjects
牛樟芝
類緣分析
生理活性
薄孔菌屬
抗癌細胞活性
Antrodia
A. cinnamomea
biological activity
anticancer activity
phylogeny
SDGs
Type
other