tudy of the Improvement of Impaired Adipose-derived Stem Cells Activities by Low-glucose
Date Issued
2012
Date
2012
Author(s)
Hsieh, Tsung-Yu
Abstract
Adipose-derived stem cells (ASCs) have been used in skin and wound repair with satisfactory results, therefore, autologous transplantation of ASCs becomes a treatment option for diabetes patients who facing the problem of delayed wound healing. However, many reports revealed that prolonged hyperglycemia damaged several organs and functions of many cell types, thus raised a concern that whether ASCs from diabetes patients suitable for medical applications. To simplify the factors involved in impaired ASCs activity and to concentrate on the results of hyperglycemia, we provided an in vitro model to investigate the effect of culture medium glucose concentration on ASCs functions during long-term culture.
We found induced cell senescence, decreased cell proliferation, migration, survival rate and response to hypoxia were displayed in ASC under high glucose condition. To further study whether these dysfunction were reversible, ASCs cultured under high glucose condition were then changed into low-glucose medium, and a significant recovery of cellular activities was observed. Collectively, these data suggested that low-glucose treatment improves the functions of ASCs impaired by high glucose concentration.
Subjects
Adipose-derived stem cells
Glucose
Hypoxia
Wound Healing
SDGs
Type
thesis
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