The Study of the RRNA Spacer Regions of the Nosema Ceranae Isolates
Date Issued
2007
Date
2007
Author(s)
Lee, Ker-Chang
DOI
zh-TW
Abstract
Since 2005, Nosema ceranae was first found in the moribund European honeybee (Apis mellifera) with nosema disease in our laboratory, this finding has evoked the worldwide reexamination of the exact caused agent of European honeybee with nosema disease. In this study, we examined the diseased European honeybees from Martiniqe Island (South America), France, Spain, and Taiwan, and also from oriental honeybee (A. cerana) from Taiwan. The results showed that only N. ceranae was detected in the collected honeybees, especially the diseased honeybees from Taiwan and Martiniqe Island (South America). In addition, no cross infection with other microsporidia was found. The ITS sequences of the N. ceranae rDNAs from the examined honey bees were highly conserved, it is suggested that this spacer sequence can be used an available marker for the detection of honey bee nosema disease. However the sequences of both IGS and SSUrRNA were polymorphic. Based on IGS sequences, there was no evolutional difference of N. ceranae from different hosts in evolution analysis, and also no allopatric differentiation occurred in the isolates from different geographic areas. N. ceranae was found in the honeybee population collected from South America, European, and Taiwan, it implied that N. ceranae has been worldwide dispersed. Furthermore, two diseased honeybees collected from France suffered from the microsporidian infection with an undefined species, it reveals that there may be more than two species of microsporidian pathogens existed in the honeybee population, so the precise identification of honeybee microsporidian pathogens is necessary. It can avoid the conflict in data caused by the misidentification of the pathogens.
Subjects
東方蜂微粒子蟲
西方蜂
東方蜂
ITS
IGS
Nosema ceranae
Apis mellifera
A. cerana
Type
thesis
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