Evaluation of hydrogen yield potential from Chlorella by photo-fermentation under diverse substrate concentration and enzyme loading
Journal
Bioresource Technology
Journal Volume
303
Date Issued
2020
Author(s)
Abstract
Chlorella is widely distributed, can be cultured in waste water and had short growth cycle. The high carbohydrate composition shows great potential for bioenergy output. In this work, concentrated Chlorella solution was adopted as raw material. Reducing sugar concentration, pH, and cumulative bio-hydrogen yield were taken as indexes, the effects of substrate concentration and enzyme (cellulase or neutral protease) load on photo-fermentation bio-hydrogen production process from microalgae biomass were investigated. Results showed that highest cumulative hydrogen yield was obtained at the optimal substrate concentration of 25 g/L, when the load of cellulase and protease are both 15%, the effect is the best which were 16.65 mL, 29.44 mL, and 43.62 mL, respectively. Results fitted well to the Gompertz model, indicating the feasibility of photo-fermentative bio-hydrogen production from concentrated Chlorella. © 2020 Elsevier Ltd
Subjects
Enzyme load; Microalgae biomass; Photo-fermentation bio-hydrogen production; Substrate concentration
Other Subjects
Algae; Biomass; Enzymes; Fermentation; Microorganisms; Substrates; Bio-hydrogen production; Carbohydrate compositions; Enzyme loads; Micro-algae; Neutral protease; Photo fermentation; Reducing sugars; Substrate concentrations; Hydrogen production; cellulase; hydrogen; neutral proteinase; sugar; hydrogen; bioenergy; biomass power; carbohydrate; concentration (composition); enzyme activity; fermentation; green alga; hydrogen; microalga; photochemistry; substrate; Article; biomass; Chlorella; concentration (parameter); fermentation; nonhuman; pH; photofermentation; priority journal; substrate concentration; fermentation; microalga; Biomass; Chlorella; Fermentation; Hydrogen; Hydrogen-Ion Concentration; Microalgae
Type
journal article