Production of Polyester Materials with Functional Groups by Fermentation
Date Issued
2005
Date
2005
Author(s)
Yang, Sheng-Pin
DOI
zh-TW
Abstract
Biosynthesis of biodegradable polymers using Pseudomonas oleovorans was conducted. Manipulations of carbon source as well as ingredients of fermentation medium were investigated. PHA (ployhydroxyalkanoate) incorporated with functional groups was anticipated. 3.38 g/L of dry cell weight and 26% of PHA content were obtained using P. oleovorans when dosing 6 g/L of sodium octanoate and 4 g/L of ammonium sulfate. 1.4 g/L of PHA were attained under a limitation of nitrogen source. PHA content was 46 % dry cell weight. If nitrogen source of medium were changed from ammonium sulfate to yeast extract, both cell concentration and weight of PHA were enhanced respectively. Fermentation with dosages of 6 g/L sodium octanoate and 16 g/L yeast extract were resulted in a high cell concentration (5.84 g/L). When dosing 8 g/L of sodium octanoate and 2 g/L of yeast extract, high PHA concentration was obtained (1.63 g/L).
Different products of PHA were obtained by dosage of different carbon sources. Both PHO (poly(3-hydroxyoctanoate)) and PHHN (poly(3-hydroxyheptanoate-co-3-hydroxynonanoate))were the major co- polymers obtained by dosaging sodium octanoate and nonanoic acid respectively as a sole carbon source. PHA contained 3-hydroxy- 10-undecenoate，3-hydroxy-7-noneneoate and 3-hydroxy-6-hepteneoate units were obtained when feeding undecylenic acid as a sole carbon source. When dosing 5-phenylvaleric acid, PHPV(Poly(3-hydroxy-5- phenylvalerate)) with phenyl group were be synthesized. Introducing the various functional groups into the structure of PHAs facilitates the development of synthesizing new functionality biopolymers for many uses.
Subjects
聚羥基烷酯
辛酸鈉
十一烯酸
苯戊酸
polyhydroxyalknoates
sodium octanoate
undecylenic acid
5-phenylvaleric acid
Type
thesis
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