Photodynamic therapy with hexa(sulfo-n-butyl)[60]fullerene against sarcoma in vitro and in vivo
Journal
Journal of Nanoscience and Nanotechnology
Journal Volume
16
Journal Issue
1
Pages
171-181
Date Issued
2016
Author(s)
Abstract
The hydrophilic molecular micellar hexa(sulfo-n-butyl)[60]fullerene (FC4S), first synthesized in 1998 as a photosensitizer (PS) has been reported to exhibit high efficacy for singlet oxygen generation and antimicrobial photodynamic inactivation. The purpose of this study was to investigate the effects of photoactivated FC4S for free radical generation and to mediate photodynamic therapy (PDT) of cancer in vitro and in vivo. The results demonstrated that following light irradiation, FC4S produced singlet oxygen, but after addition of electron donors such as ferrocytochrome c or NADH, FC4S also produced superoxide. The combination of FC4S with light irradiation was able to induce cytotoxicity to human fibrosarcoma cells and murine sarcoma 180 cells in vitro. Cell-killing was proportional to fluence as well as FC4S concentration. Photoirradiation by argon-ion laser after intraperitoneal injection of FC4S also resulted in inhibition of S180 tumor growth in vivo (up to 80% reduction of tumor volume). Hematological and blood biochemistry parameters of the cancer-bearing mice were improved by PDT. Based on these findings, we conclude that FC4S has a great potential as a nanomedicine in PDT for cancer. Copyright ? 2016 American Scientific Publishers All rights reserved.
Subjects
Hydrophilic hexa(sulfo-n-butyl)[60]fullerene (FC4S); ICR Mice; Photodynamic therapy; Reactive oxygen species; S180 murine sarcoma
SDGs
Other Subjects
Argon lasers; Diseases; Free radicals; Fullerenes; Gas generators; Hydrophilicity; Irradiation; Mammals; Medical nanotechnology; Molecular oxygen; Oxygen; Photosensitizers; Tumors; Hexa(sulfo-n-butyl)[60]fullerene; ICR Mice; Intraperitoneal injections; Photodynamic inactivation; Photodynamic therapy (PDT); Reactive oxygen species; S180 murine sarcoma; Singlet oxygen generation; Photodynamic therapy; fullerene derivative; hexasulfobutyl(60)fullerene; photosensitizing agent; animal; human; Institute for Cancer Research mouse; male; metabolism; mouse; pathology; photochemotherapy; procedures; sarcoma; Animals; Fullerenes; Humans; Male; Mice; Mice, Inbred ICR; Photochemotherapy; Photosensitizing Agents; Sarcoma
Type
journal article