Isolation and characterization of an immunomodulatory protein (APP) from the Jew’s Ear mushroom Auricularia polytricha
Resource
JOURNAL OF COLLOID & INTERFACE SCIENCE 275 (1): 309-316 JUL 1 2004
Journal
Journal of Colloid and Interface Science
Journal Volume
275
Journal Issue
1
Pages
-
Date Issued
2004
Date
2004
Author(s)
DOI
246246/2006111501275368
Abstract
A new immunomodulatory protein (APP) was purified from the fruiting body of an edible Jew’s Ear mushroom, Auricularia
polytricha, by extraction using 5% cold acetic acid in the presence of 0.1% 2-mercaptoethanol, followed by ammonium sulfate
fractionation, DE-52 and MonoQ anion-exchange chromatography. As determined by electrophoresis techniques, the molecular
mass of APP is around 13.4 kDa and its pI is approximately 5.1. APP is a simple protein without carbohydrate, and can agglutinate
mouse red blood cells. APP alone activates murine splenocytes, markedly increasing their proliferation and gamma-interferon (IFNc)
secretion, and presents no cytotoxicity in vitro. Although murine splenocytes are stimulated by the mitogen concanavalin A
(ConA), APP suppresses their proliferation in a dose-dependent manner. APP (5–20 lg/ml) also enhances the production of both
nitric oxide (NO) and tumor necrosis factor-alpha (TNF-a) by LPS-induced RAW 264.7 macrophages. These findings suggest that
APP is an immune stimulant and can strengthen the immune response of its host.
2004 Elsevier Ltd. All rights reserved.
Subjects
spherical floc
nonhomogeneous structure
two-layer model
plate
drag force
Publisher
Taipei:National Taiwan University Dept Chem Engn
Type
journal article
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