Inhalation Toxicity of Size-Segregated Ambient Particulate Matter in BALB/c Mice
Date Issued
2014
Date
2014
Author(s)
Liu, Szu-Yuan
Abstract
Evidence suggests that particulate matter (PM) is associated with cardiopulmonary effect and exacerbation of asthma. However, it is unclear whether repeated exposure to PM would cause inflammatory and immunological effects.
The aim of this study is to evaluate the effects of size-fractioned PM on lung inflammation and immune responses in healthy BALB/c mice.
We collected PM10, PM2.5, PM1 and PM0.1 from October 2012 to August 2013 at Gongguan, Taipei. These particles represented urban traffic pollution. Samples were extracted and sonicated with phosphate-buffered saline (PBS). Female BALB/c mice exposed to PM10, PM2.5, PM1, PM0.1 and PBS via intratracheal instillation with three doses of 1.75 mg/kg (35μg/per mice), 5mg/kg (100 μg/per mice), 12.5mg/kg (250 μg/per mice). After exposures, cellular profile of plasma and expression of inflammation biomarkers, immune cytokines in bronchoalveolar lavage fluid (BALF), and pulmonary responses were assessed.
PM increased inflammatory responses including neutrophils, T helper 1 cell related cytokines, TNF-α, IFN-γ. Allergic immune responses including eosinophils, T helper 2 cell related cytokines, IL-5, IL-10, IL-13, IL-17A were also increased in BALF. These inflammatory and allergic immune markers were greater in PM10 high dose group.We also found that endotoxin, Na+, Mg2+, Ca2+ and Cl- were correlated with pulmonary inflammation and immune response.
Our results indicated that exposure to particulate matter would cause allergy-like immune responses in healthy mice. However the components of PM suspension for these immune responses needs further study.
Subjects
空氣汙染
大氣懸浮微粒
肺部發炎
過敏性免疫反應
SDGs
Type
thesis
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