Three-Dimensional Cytomorphology in Fine Needle Aspiration Biopsy of Parathyroid Lesions
Resource
ACTA CYTOLOGICA v.46 n.6 pp.1037-1042
Journal
ACTA CYTOLOGICA
Journal Volume
v.46
Journal Issue
n.6
Pages
1037-1042
Date Issued
2002
Date
2002
Author(s)
CHANG, TIEN-CHUN
Abstract
OBJECTIVE: To elucidate three-dimensional (3-D) cytomorphology in fine needle aspiration biopsy (FNAB) of parathyroid lesions. STUDY DESIGN. Ultrasound-guided FNAB was performed on parathyroid lesions from 10 patients with hyperparathyroidism. The aspirates were stained and observed tinder a light microscope (LM). The aspirates were also fixed, dehydrated , critical point dried, spattered with gold ions and observed with a scanning electron microscope (SEM) . Findings under SEM were correlated with the appearances under LM as well as with serum parathyroid hormone ( PTH) concentrations. RESULTS: Under LM, nine cases displayed isokaryosis and one case, anisokaryosis. These appearances corresponded to isocytosis or anisocytosis under SEM. Under SEM, 3-D cytomorphology of parathyroid lesions displayed isocytotic, scattered cells in five cases, uniform cellular arrangements in four cases and anisocytotic, scattered cells in one case. The cell surface was rather smooth in five cases. The other five cases had significant granules on the cell surfaces; these all had serum PTH concentrations 268 pg /mL. CONCLUSION: 3-D cytomorphology in FNAB of parathyroid lesions was a rather smooth cell surface in cases with low serum PTH and a granular cell surface in cases with significantly increased serum PTH. These characteristics and the absence of microvilli might be helpful in the differential diagnosis between parathyroid and follicular thyroid lesions.
Subjects
parathyroid diseases
parathyroid hormones
aspiration biopsy
imaging
three-dimensional
scanning electron microscopy
3- DIMENSIONAL CYTOMORPHOLOGY
Type
journal article