An improved high-performance liquid chromatographic method for simultaneous determination of tocopherols, tocotrienols and γ-oryzanol in rice
Journal
Journal of Chromatography A
Journal Volume
1218
Journal Volume
1218
Journal Issue
29
Journal Issue
29
Pages
4709-4713
Start Page
4709
End Page
4713
ISSN
18733778
Date Issued
2011-07-22
Author(s)
Huang, Shao-Hua
Abstract
An improved normal phase high performance liquid chromatographic (NP-HPLC) method was developed for simultaneous quantification of eight vitamin E isomers (α-, β-, γ- and δ-tocopherols and α-, β-, γ- and δ-tocotrienols) and γ-oryzanol in rice. A complete separation of all compounds was achieved within 25min using an Inertsil® CN-3, SIL-100A 5μM (4.6mm×250mm) column and an isocratic elution system of hexane/isopropanol/ethylacetate/acetic acid (97.6:0.8:0.8:0.8, v/v/v/v) at a flow rate varying from 0.7 to 1.5mLmin-1. A linear correlation coefficient (r2>0.99) and high reproducibility were obtained at concentrations ranging 0.05-10μgmL-1 for vitamin E isomers and 0.5-500μgmL-1 for γ-oryzanol. This method proved to be rapid, accurate and reproducible.
Subjects
γ-Oryzanol
HPLC
Rice
Tocopherols
Tocotrienols
Type
journal article