Protein Aggregation Using Gold Nanoparticles and Organization between Patterned C2C12 Cells
Date Issued
2011
Date
2011
Author(s)
Chang, Chih-Chieh
Abstract
Self-organization is a common phenomenon in the nature world. In biology, in addition to individual level, such as skin patterns, self-organization procedure is generally seen in cellular and molecular levels. In cellular level, self-organization of cytoskeleton is highly connected to cell morphology, motility, and differentiation. In molecular level, protein folding is studied widely. Protein misfolding typically causes human disease such as Alzheimer’s Disease.
In our study of molecular scale, self organization of protein was evaluated by observing actin-GFP aggregation in various pH conditions. Moreover, because of the optical characteristics, gold nanoparticle is expected to be used as a sensor.
In cellular scale, substrates with various stiffness and geometries were used to evaluate the behaviors of C2C12 myoblast cells. Polyacrylamide hydrogel was used to fabricate substrates with various stiffness. Geometries were controlled using micro-contact printing with fibronectin. Self-organization of cytoskeleton in single patterned cells and connections of multiple cells were observed.
In our study, self-organizations in molecular and cellular scales were investigated. This procedure may be an approach to obtain mechanisms of disease and corresponding therapies.
Subjects
Gold nanoparticle
protein aggregation
self-organization in cells
SDGs
Type
thesis
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