Studies on the Reproductive Biology in the Freshwater Prawn Macrobrachium asperulum (Decapoda: Palaemonidae)
Date Issued
2010
Date
2010
Author(s)
Huang, Kai-Hsiang
Abstract
Reproductive biology, especially, the reproductive physiology, in Macrobrachium asperulum was investigated in the present study. The breeding period of M. asperulum is from March to September. Developing ovaries and germ cells were characterized throughout the entire ovarian cycle. In this study, differentiating germ cells were classified mainly into oogonia, primary oocytes, secondary oocytes, and mature oocytes. Secondary oocytes were further divided into five subtypes. Summarizing the above examinations and based on the relative frequency of germ cell types, six ovarian stages were identified, and M. asperulum is proposed as being a multiple spawning species. Moreover, a gradual increase in levels of the hemolymph, hepatopancreas and ovary vitellogenin-like protein were recorded during the stages I-IV. These values peaked in stage V (mature) and then declined in stage VI (spawning). After ovulation, the eggs-carrying period was about 39.17 ± 4.36 days, and the clutch size was about 86.40 ± 17.40. Egg morphology was also characterized throughout the embryogenesis.
A world wide-distributed chemical bisphenol A, an endocrine disrupter, found in the effluents of industrial and municipal sewage treatment plants, can be able to elicit the induction of biomarker vitellogenin-like protein in male M. asperulum. In addition, prawns were perceived sensitive to the estrogenic compounds.
The administration of 5 μg/g bw serotonin injection can be able to trigger ovarian development. In addition, compared to unilaterally eyetalk ablated females, bilateral eyestalk ablation had an obvious stimulatory effect on the development of ovaries, although its high mortality was recorded.
Male M. asperulum as a biomonitoring species could be considered and proposed, and levels of vitellogenin-like protein in males can be a useful biomarker of estrogenic contaminations. In addition, the biology described in present study may be beneficial in reducing hatchery man-days and the cost of production of prawn seeds. Moreover, the present study will not only integrate the field of the reproductive biology of decapod crustaceans, but also provide significant information for the future aquaculture and/or conservation of M. asperulum.
Subjects
bisphenol A
embryonic development
Macrobrachium asperulum
reproductive biology
serotonin
vitellogenesis
Type
thesis
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