Chinese olive (Canarium album L.) fruit regulates glucose utilization by activating AMP-activated protein kinase
Journal
FASEB Journal
Journal Volume
34
Journal Issue
6
Pages
7866-7884
Date Issued
2020
Author(s)
Abstract
A growing body of evidence demonstrates obesity-induced insulin resistance is associated with the development of metabolic diseases. This study was designed to investigate ethyl acetate fraction of Chinese olive fruit extract (CO-EtOAc)-mediated attenuation of obesity and hyperglycemia in a mouse model. About 60% HFD-fed mice were treated intragastrically with CO-EtOAc for last 6?weeks, and body weight, blood biochemical parameters as well as hepatic inflammation response were investigated. Our results showed that CO-EtOAc treatment significantly reduced the formation of hepatic lipid droplets, body weight gain, blood glucose, and improved serum biochemical parameters in HFD-induced obese and insulin resistant mice. We further explored the molecular mechanism underlying the blood glucose modulating effect of CO-EtOAc using L6 myotubes model. We conclude that CO-EtOAc effectively increases the glycogen content and glucose uptake by stimulating the membrane translocation of glucose transporter 4. In addition, CO-EtOAc depolarizes the mitochondrial membrane and decreases the mitochondrial oxygen consumption, which may result in AMPK activation and the consequent mitochondrial fission. This study shows that CO-EtOAc prevents the development of obesity in mice fed with HFD and is also capable of stimulating glucose uptake. The possible mechanism might be due to the effects of CO-EtOAc on activation of AMPK and promotion of mitochondrial fission. ? 2020 Federation of American Societies for Experimental Biology
Subjects
AMPK; Chinese olive (Canarium album L.); glucose uptake; high-fat diet; mitochondrial
SDGs
Other Subjects
acetic acid ethyl ester; adenine nucleotide translocase; canarium album extract; caspase 3; cholesterol; ellagic acid; ethyl gallate; gallic acid; gallic acid derivative; gallic acid methyl ester; glucose transporter 4; glycogen synthase kinase 3beta; hydroxymethylglutaryl coenzyme A reductase kinase; lactic acid; natural product; plant extract; reduced nicotinamide adenine dinucleotide dehydrogenase; succinate dehydrogenase; triacylglycerol; unclassified drug; antidiabetic agent; glucose; glucose transporter; hydroxymethylglutaryl coenzyme A reductase kinase; insulin; plant extract; animal experiment; animal tissue; Article; body weight; body weight change; Canarium album; cell differentiation; cell fractionation; confocal microscopy; controlled study; drug mechanism; enzyme activation; fasting; fruit; glucose blood level; glucose homeostasis; glucose transport; glucose utilization; glycogen analysis; histology; hyperglycemia; in vitro study; in vivo study; lipid diet; lipid liver level; liquid chromatography-mass spectrometry; live cell imaging; liver tissue; male; mitochondrial membrane potential; mitochondrial respiration; mouse; mouse model; nonhuman; obesity; olive; oxygen consumption; priority journal; protein phosphorylation; skeletal muscle cell; Taiwan; Western blotting; adverse event; animal; C57BL mouse; chemistry; drug effect; experimental diabetes mellitus; fruit; insulin resistance; liver; metabolism; obesity; olive tree; phosphorylation; physiology; AMP-Activated Protein Kinases; Animals; Blood Glucose; Body Weight; Diabetes Mellitus, Experimental; Diet, High-Fat; Fruit; Glucose; Glucose Transport Proteins, Facilitative; Hypoglycemic Agents; Insulin; Insulin Resistance; Liver; Male; Mice; Mice, Inbred C57BL; Obesity; Olea; Phosphorylation; Plant Extracts; Taiwan
Type
journal article