Evaluation on anti-microbial and anti-inflammatory effects of hops extract
Date Issued
2015
Date
2015
Author(s)
Kuo, Liang-Yu
Abstract
Hops, the female inflorescences of the hop plant (Humulus lupulus L.), are widely used in the brewing industry to add bitterness and aroma to beer. Recently, some flavanones with prenyl groups have been identified in hops and beers, and it had many biological activities. Propionibacterium acnes and Malassezia furfur play important roles in this two common skin diseases of Acne vulgaris and dandruff. The object of this study was to investigate different extracts of hop cone that effected anti-microbial and anti-inflammatory activities. In this study, LPS ( 1μg/ml)、P. acnes ( 500 μg/ml)or M. furfur (500 μg/ml) were co-treated with THP-1 cell to evaluate the production of pro-inflammatory factors. The result revealed that the lowest MIC against P. acnes was 6 μg/ml extracted by aqueous fraction of 95% aqueous ethanol (EOW) and MBC was 64 μg/ml EOW, whereas the lowest MIC against M. furfur was 1000 μg/ml extracted by ethyl acetate fraction of several solvents. In addition, 16 μg/ml of EOW and EOEA were presented significantly inhibitory abilities to nitrite oxide (NO) production (P < 0.001). All inflammatory induced group decreased the TNFα production by co-treating with 16 μg/ml EOW, but the TNFα production reduced by 8 μg/ml EOEA only in P. acnes induced group. Moreover, the concentrations to decrease IL-8 production are 16 μg/ml EOW and EOEA at LPS and P. acnes induced group, but all increased at M. furfur induced group. In conclusion, aqueous fraction of 95% aqueous ethanol (EOW) had better bioactivity for anti-microbial effect against P. acnes and alleviated the inflammatory effect.
Subjects
Humulus lupulus
anti-inflammation
anti-microbial
Malassezia furfur
Propionibacterium acnes
Type
thesis