Microbial Diversity Study of the Kuantzulingud Spring in the Southwestern Taiwan
Date Issued
2009
Date
2009
Author(s)
Lin, Yue-Ting
Abstract
Organic carbon, sulfate, methane, and hydrogen are abundant in mud springs,southwestern Taiwan. These compounds can provide the microbial community to carry on diverse metabolisms. The temperature of the Kuantzuling mud spring could be as high as 80℃, thus the mud spring may offer a great opportunity to study thermophilic microorganisms and terrestrial hot spring ecosystems. This study performed a set of enrichment experiments, stain isolations and physiological tests to understand the interaction among different microorganisms. According to the aqueous and gas chemistry data of the Kuantzuling mud spring,we designed eight types of cultural media to enrich different kinds of microbial metabolism in the slurry. They are 6 types of anaerobic media for methanogens using acetate, methanol, methylamine or hydrogen and carbon dioxide to produce methane, thiosulfate reducers using glucose, fermenters and 2 types of aerobic media for heterotrophs and methanotrophs. Once the microorganisms could adapt to the certain types of media, pure stains were tend to be isolated and tested their physiological characteristics. Microorganisms can be enriched in all types of media, but not in all cultivated temperatures. Methanogens using acetate, methanol or hydrogen and carbon dioxide ,hiosulfate reducter, fermenter, aerobic heterotroph could be enriched at temperatures higher than 50℃. Mostly of them can also be enriched at 80℃. Methanogen usingethylamine and methanotroph can only be enriched at temperature of lower than 50 ℃. Total nine types of microbial metabolisms, including sulfate reducer using lactate confirmed by previous studies, can be enriched in the Kuantzuling mud spring. This result is consistent with previous energetic evaluation of microbial metabolisms andolecular analysis of the slurry. It also inferred that the microorganisms exhibited a great diversity of metabolic pathways and were either competitive or beneficial to eachthers while processing organic carbon degradation or carbon cycling. Strain KTL01-S-121106-I1 and KTL01-S-121106-I2 were isolated from the Kuantzuling mud spring with heterotrophy media. The 16S rDNA gene sequence of strain I1 exhibited a very close affiliation (at a similarity of 99%) with Meiothermus ruber strain SPS242. It can grow at the pH between 5 and 9, in the temperature ranging from 20 to 70℃ with the optimal growth temperature at 60℃. The 16S rDNAene sequence of strain I2 was close to that of Rhodobacter vinaykumarii JA123 at the similarity of 95%, but stain I2 is not phototroph and probably is a new species. Strain2 can grow at the pH between 6 and 9, in the temperature ranging from 20 to 60℃ with the optimal growth temperature at 50℃.
Subjects
Kuantzuling
mud spring
thermophilic microorganism
diversity of
SDGs
Type
thesis
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