Analysis of cDNA Sequence and Regulation of mRNA Levels of Pituitary Glycoprotein Hormones in The Chinese Soft-shell Turtle (Pelodiscus sinensis)
Date Issued
2004
Date
2004
Author(s)
Chien, Jung-Tsun
DOI
zh-TW
Abstract
The pituitary glycoprotein hormones (thyrotropin, TSH; follitropin, FSH; lutropin, LH) are composed of two dissimilar subunit molecules, alpha and beta subunits are identical in a species; while beta subunits are specific and determine the hormone activity and species specificity. The comparative studies of cloning of cDNAs encoding these hormones and their gene regulations have been extensively conducted in mammals, birds, amphibian and fish. However, virtually no information has been available in reptiles. Reptiles occupy a key position in evolutionary history of vertebrates as the birds and mammals evolved from them. For better understanding the phylogenetic diversity and evolution of the cDNA nucleotide and peptide sequences of pituitary glycoprotein hormones in vertebrates, a study was conducted to clone the cDNAs of alpha subunit and the beta subunits of TSH, LH, and FSH of the Chinese soft-shell turtle, Pelodiscus sinensis, and to investigate the regulation of gene expression. The cDNAs were cloned by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) methods. The cloned alpha cDNA consists of 670-bp nucleotides, including 71-bp 5’-untranslated region (UTR), 363-bp open reading frame, and 3’-UTR of 219-bp nucleotides, following by a 17-bp poly A trait. It encodes a 120 amino acid precursor molecule of alpha subunit with a putative signal peptide of 24 amino acids and a mature protein of 96 amino acids. The cloned TSH beta cDNA consists of 580-bp nucleotides, including 67-bp of 5’UTR, 402-bp of the open reading frame, and 97-bp of 3’UTR followed by a 14 bp poly A trait. It encodes a putative precursor protein of 133 amino acids with signal peptide of 19 amino acids and a mature protein of 114 amino acids. The cloned FSH
Subjects
甲釋素
性釋素
基因調控
基因選殖
次單元
甲狀腺素
演化
歧異度
腦垂體
糖蛋白激素
促甲狀腺激素
促濾胞激素
促黃體激素
GnRH
gene expression and regulation
subunit
diversity
evolution
lutropin
TRH
thyroid hormone
phylogenetic tree
thyrotropin
follitropin
pituitary glycoprotein hormone
sinensis
Chinese soft-shell turtle
Pleodiscus
Type
other
File(s)![Thumbnail Image]()
Loading...
Name
ntu-93-D84225011-1.pdf
Size
23.31 KB
Format
Adobe PDF
Checksum
(MD5):a6f263154aa830b5e1d8c4f207b56f9c