The study of the effects of biodegradable polymers and ECM proteins on chondrocyte adhesion, proliferation and gene expression
Date Issued
2004
Date
2004
Author(s)
Chen, Chung-Hong
DOI
zh-TW
Abstract
The goal of this study was to compare the performance of chondro- cytes in adhesion, proliferation and gene expression of chondrocytes when they were cultivated on different biodegradable polymers. Four kinds of biodegradable polymers was used in this study, including PDLLA, PLLA, PLGA, and PCL, which were made by using the solvent- casting method, and chondrocytes were cultured on the polymer films. Cell adhesion on day 1, cell proliferation and gene expression on day 4 and day 7 were investigated. The results show that chondrocytes prolife- rated better on PLGA and PDLLA films in 7 days, but there are no signi- ficant differences on each film for 14 days. The gene expression on diffe- rent polymer films showd difference. We suggest the PDLLA is most suitable for chondrocyte culture, since the gene expression of chondro- cytes is restored during 14 days culture period.
As a result, PDLLA films was modified by adsorption of collagen type I, collagen type II, fibronectin, and polylysine. Cell adhesion on day 1, cell proliferation and gene expression on day 4,7,14 were investigated. The results showed that collagen type I, collagen type II, and fibronectin could enhance chondrocytes proliferation during culture. The gene expression was retained during 14 days on each surface. It seems that the adsorbed proteins would not effect the gene expression of chondrocytes.
Subjects
細胞外間質蛋白質
軟骨細胞
貼附
基因表現
ECM protein
chondrocyte
phenotype
adhesion
Type
thesis
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