Effect of Infrared Radiation on Rat PC12 Cells Growth and Expression and MEA chip
Date Issued
2010
Date
2010
Author(s)
Weng, Yu-Fu
Abstract
The purpose of this study is to simulate the rat neuron cell growth and protein expression in infrared radiation, and design a machine which combine the infrared emitter and carbon dioxide incubator, and utilize the above cells to simulate the rat neuron cells, and to know the cell growth by observing the cell coverage and cell numbers. After 4 days exposure to infrared radiation, the cell coverage and cell numbers of PC12 and PC12 which treated with nerve growth were measured. Two dimensional protein gel electrophoresis and mass spectrometry are also applied to observe the protein expression pattern. From the results, the cell growth and protein quality under infrared radiation are changed. The difference is visible in infrared radiation 1 day. We also design the neuron chip to measure the neuron electric signals.
Subjects
PC12
MEA chip
Type
thesis
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