Real-time second-harmonic-generation microscopy based on a 2-GHz repetition rate Ti:sapphire laser
Resource
Optics Express 11 (8): 933-938
Journal
Optics Express
Journal Volume
11
Journal Issue
8
Pages
933-938
Date Issued
2003
Author(s)
Chu, S.-W.
Liu, T.-M.
Sun, C.-K.
Lin, C.-Y.
Tsai, H.-J.
Liu, Tzu-Ming
Sun, Chi-Kuang
Lin, Cheng-Yung
Tsai, Huai-Jen
Abstract
The problem of weak harmonic generation signal intensity limited by photodamage probability in optical microscopy and spectroscopy could be resolved by increasing the repetition rate of the excitation light source. Here we demonstrate the first photomultiplier-based real-time second-harmonic-generation microscopy taking advantage of the strongly enhanced nonlinear signal from a high-repetition-rate Ti:sapphire laser. We also demonstrate that the photodamage possibility in common biological tissues can be efficiently reduced with this high repetition rate laser at a much higher average power level compared to the commonly used ~80- MHz repetition rate lasers.
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Type
journal article
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