Variations among populations of Aedes aegypti in Taiwan: based on tergum white-scale patterns, n-alkane hydrocarbons and random amplified polymorphism DNA of adults Aedes aegypti
Date Issued
2005
Date
2005
Author(s)
Su, Yu-Cheng
DOI
zh-TW
Abstract
There are some variation, in the distribution of white scale on the abdominal tergites of Aedes aegypti. The C. K.Meek’s (CKM) classification system was used to identify and rank eight classes in the abdominal scale patterns, including CKM0 which was generalized as the dark form of Ae. aegypti formosus, CKM1 as the type form of Ae. aegypti aegypti and CKM2-7 as the other pale form of Ae. aegypti aegypti. The RAPD fragments profiles of seven CKM laboratory selected strains were analyzed and genetic similarities were estimated, the UPGMA dendrogram assorted these strains into two major groups: the type form and the pale form. The dendrogram was detected based on n-alkane cuticular hydrocarbon profiles of seven CKM strains. All nineteen n-alkanes (n-C18 to n-C36) were shared from female adult’s epicuticle among seven CKM strains, but varied quantitatively. The mosquito with CKM values of 2-7 strains had more abundance of components n-C18 to n-C27 than CKM 1, less abundance of n-C29 to n-C36.
The scale patterns of field collected Ae. aegypti were found to have the type form and the pale form, those were mixed in the similar niche in Taiwan. The female mosquitoes within populations exhibited greater variation in scale pattern than males. The frequency CKM value in female individual was used for cluster analyses, that generated a dendrogram depicting the branches of populations. The nine populations assorted into two clusters that correspond geographically with southwestern and southeastern area in Taiwan. We also found that the southeastern mosquito populations exhibited high frequency of pale form than that of southwestern. The similar phenomenon was observed in indoor breeding populations than outdoors. Therefore, the cuticular n-alkane hydrocarbons of adults from southeastern Taiwan and indoor breeding populations were analyzed, we found these mosquitoes contained larger amount of n-C18 to n-C27. Nineteen types of n-alkanes were identified using gas chromatography electron impact-mass spectrometry. Dendrogram obtained from cluster analysis showed that grouping associated on the frequencies of pale form but not with geographic distribution. Differences in the frequencies of RAPD markers were detected among populations. Cluster analyses revealed, there were two groups separated by the Central Mountain Range. Regression analysis of geographic distances and pairwise Fst values estimated from RAPD markers showed that southwestern populations were isolated by distance of 33 km within southwestern populations and 29 km within southeastern in Taiwan.
Subjects
白鱗分布
埃及斑蚊
RAPD
表皮正烷類
族群差異
scale pattern
cuticular n-alkanes
Aedes aegypti
Type
thesis
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