Inflammatory cytokines induce CCL20 expression caused lymphocytes recruitment or promote oral cancer cells migration
Date Issued
2015
Date
2015
Author(s)
Su, Kuan-Wei
Abstract
CCL20 is a member of the CC-chemokine family and its sole receptor is known as CC chemokine receptor 6 (CCR6). There were elevated CCL20 expression reported in variety of human cancers, which may contributed to inflammatory microenvironment in tumor site. Interestingly, there is a unique subtype of regulatory T cells (Tregs) which specifically express CCR6. Admittedly, CCR6+ Foxp3+Tregs can be attracted by CCL20 to the tumor site. This phenomenon may cause immunity suppression, hence promotes tumor progression. Furthermore, CCL20 also known to attract lymphocytes which express CCR6. α-enolase (ENO1) is an enzyme often overexpressed in various cancer, and it is positively correlated to CCL20 expression. In addition, CCL20/CCR6 interaction could increase the migration ability in some cancers. This study aimed to investigate the relationship between CCL20, Foxp3+ regulatory T cells, CD8+ T cells, ENO1 and clinical characteristics of oral cancer patients. We also aimed to study the effect of CCL20 on migration ability of oral cancer cell lines. In present study, we collected clinical oral cancer patients and performed immunohistochemistry staining of CCL20 and Foxp3. Moreover, we found that LPS and TNF-α, represented as inflammatory stimuli, could stimulate CCL20 expressing and releasing in human oral cancer cell lines: SAS, HSC-3 and Ca9-22 cells. Furthermore, we found that oral cancer cell lines had chemotactic response to CCL20. Due to CCL20 and CCR6 are the only ligand and receptor for each other, it is innovative to understand their role in oral cancer progression.
Subjects
inflammatory cytokines
migration
oral cancer
SDGs
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-104-R02450016-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):b613798a8c59391526928025cd6d701a