Effects of Grafting of Functional Polymers to Substrates on Cell Culture
Date Issued
2014
Date
2014
Author(s)
Fu, Szu-Wei
Abstract
In surface modification of biomaterials, grafting functional polymers to substrates is one strategy for tailoring surface property to meet the particular demand. It is various approach of conjugation and functional polymers lead to a diversity of surface modification. Here, we try to develop the following two kinds of surface modification.
In the first part of study, by a UV-activated azide-based crosslinking mechanism, we immobilized thermo-responsive polymer poly(N-isopropylacrylamide) (PNIPAAm) on plastic substrate. Cell sheets could be harvested by temperature change with an intact extracellular matrix. Next, grooved PNIPAAm substrates were fabricated by imprinting from grooved poly(dimethylsiloxane) (PDMS) molds (800 nm in groove width and 500 nm in depth). C2C12 cells formed aligned cell sheets on the grooved PNIPAAm surface. The aligned cell sheet could be transferred to a gelatin substrate without losing cell alignment.
In the second part of study, we deposited double bond-containing maleimide onto substrate by chemical vapor deposition in a substrate independent manner. Also, thiol containing cysteamine were copolymerized with zwitterionic sulfobetaine methacrylate (SBMA) in different molar ratio, and surfaces were modified via thio-ene reaction. Thanks to the superhydrophilicity of SBMA, the modified surfaces possessed good efficacy of anti-fouling. Among the monomer ratios tested in the experiment, the amount of cell adhesion, platelet adhesion and protein adsorption were decreased more profoundly on the surface modified with polymer with higher thiol groups on the side chain.
Subjects
表面改質
疊氮基交聯
聚(N-異丙基丙烯酰胺)
細胞薄片
化學氣相沉積
硫醇-雙鍵反應
磺基甜菜鹼甲基丙烯酸酯
抗貼附
Type
thesis
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