The Effect of Glutathione-Supplemented Hyaluronan on Osteoarthritis Synoviocytes
Date Issued
2011
Date
2011
Author(s)
Chen, Wei-Yu
Abstract
Osteoarthritis (OA) is recently recognized as a “non-classical” inflammatory disease. Glutathione (GSH), an antioxidant, has been shown to have an anti-inflammatory effect on protecting cells from reactive oxygen species such as free radicals and peroxides. This study is to examine the effects of hyaluronic acid (HA) supplemented with GSH on expression of four OA-related factors in fibroblast-like synoviocytes (FLSs) from five patients with OA. 0.1mg/ml of HA (Ostenil®) supplemented with different weight percent (5%, 10% and 20%) GSH were co-cultured with FLSs. The gene expression and protein production of OA-related factors, such as TNF-α, IL-6, MMP-3 and TIMP-1 were evaluated by real-time quantitative PCR. The gene expression of TNF-α and MMP-3 were found decreased significantly in every group. TIMP-1 was increased when HA supplemented with GSH in weight percent 5% and 20%. HA supplemented with GSH in weight percent 20% has showed significantly effect on regulating these factors. It had attenuated TNF-α, MMP-3 and up-regulated TIMP-1, IL-6 mRNA expression. We suggest that intra-articular injection of HA supplemented with GSH may be feasible for relieve symptoms of knee osteoarthritis.
Subjects
Osteoarthritis (OA)
Hyaluronic acid (HA)
Glutathione (GSH)
Fibroblast-like synoviocytes (FLSs)
Type
thesis
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