Single-cell signaling network profiling during redox stress reveals dynamic redox regulation in immune cells.
Journal
Nature communications
Journal Volume
16
Journal Issue
1
Start Page
Article number 5600
ISSN
2041-1723
Date Issued
2025-07-01
Author(s)
Wang, Yi-Chuan
Wu, Ping-Hsun
Ting, Wen-Chieh
Wang, Yi-Fu
Yang, Ming-Han
Su, Jia-Ying
Sun, Hsun-I
Huang, Wei-Min
Tsai, Pei-Ling
Wernig, Gerlinde
Ho, Ping-Chih
Wang, Limei
Meng, Tzu-Ching
Chang, Yao-Ming
Lai, Shih-Lei
Li, Chia-Wei
Ko, Tai-Ming
Chang, Ya-Jen
Chern, Yijuang
Kuo, Mei-Chuan
Huang, Yen-Tsung
Tzeng, Yi-Shiuan
Chen, Shih-Yu
Abstract
In eukaryotic cells, reactive oxygen species (ROS) serve as crucial signaling components. ROS are potentially toxic, so constant adjustments are needed to maintain cellular health. Here we describe a single-cell, mass cytometry-based method that we call signaling network under redox stress profiling (SN-ROP) to monitor dynamic changes in redox-related pathways during redox stress. SN-ROP quantifies ROS transporters, enzymes, oxidative stress products and associated signaling pathways to provide information on cellular redox regulation. Applied to diverse cell types and conditions, SN-ROP reveals unique redox patterns and dynamics including coordinated shifts in CD8 T cells upon antigen stimulation as well as variations in CAR-T cell persistence. Furthermore, SN-ROP analysis uncovers environmental factors such as hypoxia and T cell exhaustion for influencing redox balance, and also reveals distinct features in patients on hemodialysis. Our findings thus support the use of SN-ROP to elucidate intricate redox networks and their implications in immune cell function and disease.
Type
journal article