SiRNA and paclitaxel loaded PEG-PCL-PEI tri-block polymeric micelle for gene and chemo-therapy in multidrug-resistance cancer cells
Journal
2011 NSTI Nanotechnology Conference and Expo
Journal Volume
3
Pages
225-228
Date Issued
2011
Author(s)
Abstract
An annoying problem of chemotherapy is the drug resistance of cancer cells which express of multidrug-resistance associated proteins and thereby resulting in ineffective treatment. To conquer this problem, tri-block copolymer, polyethylene glycol-polycaprolactone-polyethylenimine forming micelle was utilized to encapsulate the chemotherapeutic drug, paclitaxel (PTX), in the core of micelle and to attract the small interfering RNA (siRNA) on the corona of micelle and to make drug resistance cancer cell sensitizing to chemotherapeutic drug. Gratifyingly, dual agents (PTX and siRNA) combination polymeric micelles were showed to increase the intracellular accumulation of paclitaxel and were observed to kill doxorubicin resistant (MCF-7 ADR) cells by silencing the expression of multidrug-resistance associated proteins, P-glycoprotein. This result suggested that the tri-block polymeric micelle as a potential carrier for gene and chemotherapy against the multidrug-resistance cancer cell.
Subjects
Chemotherapy; Gene therapy; Micelle; Multidrug-resistance
SDGs
Other Subjects
Cancer cells; Chemotherapeutic drugs; Doxorubicin; Drug resistance; Intracellular accumulation; Multidrug-resistance; P-glycoprotein; Paclitaxel; Polymeric micelle; Small interfering rna (siRNA); Block copolymers; Cells; Chemotherapy; Diseases; Fluidics; Functional polymers; Glycols; Glycoproteins; Medical nanotechnology; Micelles; Nanotechnology; Pharmacodynamics; Polycaprolactone; Polyethylene glycols; RNA; Gene therapy
Type
conference paper