Functional and Genetic Analysis of a Proapoptotic Gene eif-3.k in Caenorhabditis elegans
Date Issued
2006
Date
2006
Author(s)
Wu, Shu-Chun
DOI
en-US
Abstract
Programmed cell death is important for development of animal. EIF3K was identified as DAPK interacting protein by using the death domain of DAPK as a bait in a yeast two-hybrid. EIF3K is highly conserved in metazoan. We found that EIF-3.K, the EIF3K homolog in C. elegans, had a pro-apoptotic activity. An eif-3.k mutation had reduced cell corpses during embryogenesis, whereas overexpression of eif-3.k caused extra cell deaths. Human EIF3K was predicated to contain two domains: HAM and WH. We performed deletion assays and found that HAM domain is dispensable for the pro-apoptotic function of EIF-3.K, but WH domain is necessary for its function. Furthermore, we found that expression of the WH domain also was sufficient to rescue the cell-death phenotype of the eif-3.k mutants. As no direct interaction was found between EIF-3.K and previously characterized components of the apoptotic machinery, such as CED-3, CED-4, and DAPK-1, we sought to identify proteins that interact with EIF-3.k by a yeast two-hybrid screen. We isolated three potential candidate clones: C17G10.9, NCL-1, and CEH-26. Only mutation in ceh-26 but not other two genes decreased number of cell corpses. Further genetic studies suggested ceh-26 and eif-3.k may participate in the same pathway during programmed cell death.
Subjects
線蟲
細胞死亡
cell death
apoptosis
eif-3.k
C.elegans
Type
other
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