Rapid characterization of sugar-binding specificity by in-solution proximity binding with photosensitizers
Resource
GLYCOBIOLOGY, 21(7), 895-902
Journal
Glycobiology
Pages
895-902
Date Issued
2011
Date
2011
Author(s)
Chang, Chuan-Fa
Pan, Jia-Fu
Lin, Chun-Nan
Wu, I-Lin
Wong, Chi-Huey
Lin, Chun-Hung
Abstract
Cell-surface carbohydrates are known to participate in many important physiological and pathological activities by interacting with their corresponding proteins or receptors. Although several methods have been developed for studying carbohydrate-protein interactions, one major problem originates from the weak bindings of carbohydrates/proteins that are often lost during repeating wash steps. Herein, we established a homogeneous solution carbohydrate array in which polyacrylamide-based glycans are used for offering a multivalent environment. The method requires no wash step and can be carried out in a high-throughput manner. We characterized the carbohydrate-binding specificities of 11 lectins and 7 antibodies, the majority of which displayed the binding patterns in consistence with previous reports. These results demonstrate that our developed solution carbohydrate array provides a useful alternative that is better than or comparable with the current available methods.
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Type
journal article
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