Immunohistochemical Study of Cadherin-Catenin Complex on Thyroid Neoplastic Tissue
Date Issued
1999-07-31
Date
1999-07-31
Author(s)
黃實宏
DOI
882314B002297
Abstract
E-cadherin is a member of the cadherin
family that plays a major role in epithelial integrity
and tumorogenesis. Catenins are a group of
cytoplasmic proteins that regulate the intracellular
anchorage of cadherin and are required for the
linkage between cadherin and the actin
cytoskeleton. Loss of E-cadherin contributes to the
pathogenesis in tumor invasion and gives a poor
prognosis. In order to investigate the adhesion
property of intercellular junctions in thyroid
tumors, expression of a-, b-, and g-catenin should
also be studied. A correlation between these
molecular markers and malignancy would be
useful as a preoperative diagnostic test for thyroid
neoplasms. The expression of E-cadherin, a, b,
and g-catenin were studied in normal and
neoplastic thyroid tissue by immunofluorescence
microscopy and Western blot analysis. In the
normal thyroid and in nodular goiter, and follicular
adenoma, staining for E-cadherin, a-, b-, and g-
catenin was seen mainly at the lateral surface of
epithelial cells in the follicle and the presence of
these molecules was confirmed by Western blot
analysis. Follicular carcinoma tissue stained
positive for E-cadherin and a-catenin, but the
results of b- and g-catenin immunostaining were
highly variable, with b-catenin being absent in
most follicular carcinomas (8/10) and g-catenin
being absent in some follicular carcinomas (3/10).
These results suggest that E-cadherin expression
was not reduced during the pathogenesis of
differentiated thyroid malignancies. Impairment of
the cadherin-catenin complex at the cell junction
may contribute to the malignant progression of
differentiated thyroid neoplastic tissue.
Subjects
cadherin
catenins
thyroid carcinoma cell
Publisher
臺北市：國立臺灣大學醫學院外科
Type
report
File(s)![Thumbnail Image]()
Loading...
Name
882314B002297.pdf
Size
44.71 KB
Format
Adobe PDF
Checksum
(MD5):c99bd7dbe2b1ce09518bf0d931cb9a8b