Simultaneous determination of sweeteners and preservatives in preserved fruits by micellar electrokinetic capillary chromatography
Journal
Journal of Chromatographic Science
Journal Volume
38
Journal Issue
8
Pages
345-352
Date Issued
2000
Author(s)
Abstract
A micellar electrokinetic capillary method for the simultaneous determination of the sweeteners dulcin, aspartame, saccharin, and acesulfame-K and the preservatives sorbic acid; benzoic acid; sodium dehydroacetate; and methyl-, ethyl-, propyl-, isopropyl-, butyl-, and isobutyl-p-hydroxybenzoate in preserved fruits is developed. These additives are ion-paired and extracted using sonication followed by solid-phase extraction from the sample. Separation is achieved using a 57-cm fused-silica capillary with a buffer comprised of 0.05M sodium deoxycholate, 0.02M borate-phosphate buffer (pH 8.6), and 5% acetonitrile, and the wavelength for detection is 214 nm. The average recovery rate for all sweeteners and preservatives is approximately 90% with good reproducibility, and the detection limits range from 10 to 25 μg/g. Fifty preserved fruit samples are analyzed for the content of sweeteners and preservatives. The sweeteners found in 28 samples was aspartame (0.17-11.59 g/kg) or saccharin (0.09-5.64 g/kg). Benzoic acid (0.02-1.72 g/kg) and sorbic acid (0.27-1.15 g/kg) were found as preservatives in 29 samples.
Other Subjects
4 hydroxybenzoic acid; acesulfame; acetonitrile; aspartame; benzoic acid; boric acid; buffer; butyl paraben; dehydroacetic acid; deoxycholate sodium; ethyl paraben; food preservative; isobutyl paraben; isopropyl paraben; methyl paraben; phenethylurea; phosphate; propyl paraben; saccharin; sorbic acid; sweetening agent; unclassified drug; article; food analysis; food preservation; micellar electrokinetic chromatography; reproducibility; solid phase extraction; ultrasound; Acetonitriles; Buffers; Chromatography, Micellar Electrokinetic Capillary; Food Preservation; Food Preservatives; Fruit; Hydrogen-Ion Concentration; Methanol; Sweetening Agents
Type
journal article