Androgen receptor regulates expression of skeletal muscle-specific proteins and muscle cell types
Journal
Endocrine
Journal Volume
25
Journal Issue
1
Pages
27-32
Date Issued
2004
Author(s)
Altuwaijri S.
Dong K.L.
Chuang K.-H.
Ting H.-J.
Yang Z.
Xu Q.
Tsai M.-Y.
Yeh S.
Hanchett L.A.
Chang C.
Abstract
C2C12 myoblasts expressing the androgen receptor (AR) were used to analyze the role of androgen-AR signaling pathway in skeletal muscle development. Marked up-regulation of AR expression was observed in differentiated myotubes. A nuclear run-on transcription assay demonstrated that transcription of the AR gene is increased during skeletal muscle cell differentiation. Regulation of skeletal muscle-specific protein expression by the androgen-AR signaling pathway was further analyzed using quadriceps skeletal muscle from wild-type (WT) and AR knock-out (ARKO) male mice. A histological analysis of quadriceps skeletal muscle indicates no morphological differences between ARKO and WT mice. However, the androgen-AR signaling pathway increases expression of slow-twitch-specific skeletal muscle proteins and downregulates fast-twitch-specific skeletal muscle proteins, resulting in an increase of slow-twitch muscle fiber type cells in quadriceps muscle.
Type
journal article