Surface Modification of Polydimethylsiloxane (PDMS) with Silanated Hydrophilic Polymer to Reduce Nonspecific Protein Adsorption
Date Issued
2015
Date
2015
Author(s)
Lin, Yu-Jou
Abstract
Glaucoma, an irreversible damage in eye diseases, usually causes blindness. Several studies have shown that intraocular pressure (IOP) is a major risk factor for optic nerve damage; therefore, the main goal of glaucoma treatment usually focuses on how to reduce IOP sufficiently in order to suppress glaucoma progression. Recently, glaucoma drainage implant (GDI) surgery becomes the main treatment of glaucoma due to its effect on prevention of glaucoma. However, the surgery wound will cause function loss of GDI because the wound healing process will result in protein adsorption, fibroblast adhesion, and proliferation. In this study we modified polydimethylsiloxane (PDMS) surfaces to reduce nonspecific protein adsorption by three hydrophilic polymers. We designed a two steps surface modification method: First, synthesis of silanated hydrophilic polymer and then grafting it onto a PDMS surface. The modified surface was analyzed by surface characterization tests such as contact angle goniometry, attentuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS). Protein adsorption, platelet adhesion and cell adhesion test were also used to examine the biological effects of modified surfaces. Our results showed that the modification of PDMS surfaces was successful and the modified surfaces significantly reduced protein adsorption, platelet adhesion and cell adhesion.
Subjects
Polydimethylsiloxane (PDMS)
nonspecific protein adsorption
surface modification
radical polymerization
zwitterionic molecule
hydrophilic
Type
thesis
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