Effect of heat pre-treatment temperature on isolation of hydrogen producing functional consortium from soil
Resource
RENEWABLE ENERGY, 35(12), 2649-2655
Journal
Renewable Energy
Pages
2649-2655
Date Issued
2010
Date
2010
Author(s)
Ravindran, Anita
Adav, Sunil
Yang, Shang-Shyng
Abstract
A functional hydrogen producing consortium was isolated from soil by heat pre-treatment technique and hydrogen production at different substrate concentration was evaluated. The forest soil was heat pre-treated at 65, 80, 95, 105 and 120 °C temperature for 1 h. As revealed by PCR-DGGE analysis and hydrogen yield, the hydrogen producing microbial community changed with increase in heat pre-treatment temperatures giving potential hydrogen producing consortium at 95-105 °C soil pre-treatment. The maximum hydrogen production rate, hydrogen yield and cumulative hydrogen with 15-20 g glucose were 1390-1576 mL/L/day, 1.83-1.93 mol H2/mol glucose, and 2966-3146 mL H2/L, respectively. The metabolic pathways shifted from ethanol-type to acetate-formate type as soil pre-treatment temperature increased from 65 to 120 °C. The soil heat pre-treatment approach is effective for isolating hydrogen producing natural Clostridium consortium from the soil as enumerations of the functional strains need specific temperature range to florish. © 2010 Elsevier Ltd.
Subjects
Forest soil; Functional consortium; Heat pre-treatment; Hydrogen production
Other Subjects
AS-soils; Different substrates; Forest soils; Functional consortium; Functional strain; Hydrogen production rate; Hydrogen yields; Metabolic pathways; Microbial communities; PCR-DGGE; Pre-Treatment; Pretreatment temperature; Temperature range; Ethanol; Gas producers; Glucose; Soils; Hydrogen production; bacterium; forest soil; heating; hydrogen; metabolism; microbial community; polymerase chain reaction; soil microorganism; temperature effect; Clostridium
Type
journal article
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