酵母菌減數分裂中粗絲期檢控機制之研究
Date Issued
2005
Date
2005
Author(s)
DOI
922311B002102
Abstract
Meiosis is essential for sexual reproduction. To ensure the success of meiosis,
meiotic checkpoints operate to coordinate the proper order of meiotic events.
In particular, the pachytene checkpoint prevents exit from the pachytene stage
of meiotic prophase when meiotic recombination and chromosome synapsis
are incomplete. Our research is planned to study the molecular mechanism of
the pachytene checkpoint in detail.
In budding yeast Saccharomyces cerevisiae, the NDT80 gene encodes a
meiosis-specific transcription activator that is required for progression from
pachytene into meiosis I. Multiple phosphorylation of Ndt80 occur in
wild-type cells, but it is inhibited in cells arrested at the pachytene stage by
the pachytene checkpoint. Based on these results, we suggested that Ndt80 is a
direct target of the pachytene checkpoint. On the other hand, other reports
proposed that Ndt80 activity is regulated indirectly at a transcriptional level
by the pachytene checkpoint through the Sum1 transcription repressor.
In our studies on defining Ndt80 functional domains, we have isolated a
dominant deletion mutation, NDT80-bc. In this study, we have confirmed that
the Ndt80-bc protein is resistant to the control of the pachytene checkpoint. It
bypassed all the pachytene-arrested mutants tested, and it did not restore spore
viability and crossover frequency. We proposed that the Ndt80 protein itself is
a direct target of the pachytene checkpoint. The deleted region from Ndt80-bc
might be the interacting site with an unidentified checkpoint protein(s). The
Ndt80-bc provides a good evidence for our hypothesis and points out an
important direction for the studies on pachytene checkpoint.
The information learned from this project will provide valuable information
for the understanding of meiotic cell-cycle control. It is also useful and applicable to researches on mitotic cell cycle, human genetic diseases, and cancers.
Subjects
meiosis
cell cycle
pachytene checkpoint
budding yeast
SDGs
Publisher
臺北市：國立臺灣大學分子與細胞生物學研究所
Type
report
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