Functional studies of FIN219 and PHYA interaction inrabidopsis thaliana
Date Issued
2008
Date
2008
Author(s)
Chao, Li-Ya
Abstract
Light is one of the most important enviroment factors, and plants have evolved complex and sophisticated mechanisms to adjust their development to fit this factor. Phytochrome A (phyA) is one of the most extensively studied photoreceptors. phyA mainly absords far-red light (FR,wavelength 700~750nm) in Arabidopsis. FAR-RED INSENSITIVE 219 (FIN219) belongso a GH3-like gene family in Arabidopsis and can be rapidly induced by auxin. There are two coiled-coil domains in FIN219 located in the N- and C-terminal regions respectively. Previous results from yeast two-hybrid studies revealed that the N- and C-terminal domains of FIN219 can interactith the full length of PHYA. Thus, this thesis will confirm the interaction between FIN219 and PHYA, and investigate their regulatory relationship. Co-immunoprecipitation studies indicate that FIN219 can interact with PHYA in the dark and far-red light. Epistatic analysis reveals that FIN219unctions downstream of the photoreceptor phyA. Western blot results showed that FIN219 protein accumulation is positively regulated by PHYA under darkness and far-red light. FIN219 promoter::GUS activity assay in phyA (pFIN219::GUS/phyA) or wild type (pFIN219::GUS/Col-0) under darkness, white light and far-red light revealed that GUS staining is almostbolished in the phyA mutant compared to that in wild type. In addition subcellular localization studies reveal that GFP-FIN219 can colocalize with PHYA-DsRed under darkness and far-red light but FIN219 is not specifically required for light-regulated nuclear accumulation of phyA.
Subjects
FIN219
PHYA
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