Rhodostomin Inhibits the Transforming Growth Factor-Beta1- Enhanced Adhesion
Resource
TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE v.191(3) pp.145-55
Journal
TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE
Journal Volume
v.191(3)
Pages
145-55
Date Issued
2000
Date
2000
Author(s)
YANG, RONG-SEN
HUANG, TUR-FU
Abstract
We have investigated the effect of transforming growth factor-beta1 (TGF- beta1) on the in vitro adhesion activity of the rat osteosarcoma cell lines (ROS 17/2.8) to extracellular matrix substrata, including fibronectin, type I and IV collagen, as well as laminin. The interaction of Arg-Gly-Asp (RGD) and rhodostomin, an RGD containing snake venom, with TGF-beta1 on the cell adhesion was also evaluated. The results showed that incubation with various concentration of TGF-beta1 (1-15 ng/ml) significantly increased the adhesion activity (1.4 to 2.5 folds) of ROS 17 /2.8 to fibronectin and type I collagen (p<0.01), whereas the adhesion activity to laminin and type IV collagen was slightly elevated (1.1 to 1.5 folds). The peak effect of TGF -beta1 on the cell adhesion occurred after pretreatment of ROS 17/2.8 with TGF-beta1 for 6 hours. Treatment with Arg - Gly-Asp-Ser (RGDS) and rhodostomin effectively suppressed the TGF-beta1- enhanced adhesion activity to fibronectin and type I collagen. This study demonstrated that the up- regulated cell adhesion activity of ROS 17/2.8 cells by the TGF-beta1 can be inhibited by the rhodostomin.